Biochemical methods to assess CFTR expression and membrane localization.

Carlos M Farinha; Deborah Penque; Mónica Roxo-Rosa; Gergely Lukacs; Robert Dormer; Margaret McPherson; Malcolm Pereira; Alice G M Bot; Huub Jorna; Rob Willemsen; Hugo Dejonge; Ghanshyam D Heda; Christopher R Marino; Pascale Fanen; Alexandre Hinzpeter; Joanna Lipecka; Janine Fritsch; Martina Gentzsch; Aleksander Edelman; Margarida D Amaral

doi:10.1016/j.jcf.2004.05.017

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Biochemical methods to assess CFTR expression and membrane localization.

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Abstract

Detection of cystic fibrosis transmembrane conductance regulator (CFTR) protein is usually a difficult task to accomplish due to the low levels of expression and high turnover that this membrane protein is submitted to in the cell. Common biochemical methods can be used for the detection of CFTR but several critical points must be taken into account. The scope of this article is to outline biochemical methods commonly used to assess CFTR expression, processing and membrane localization.

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Biotinylation Cell Membrane Cystic Fibrosis Transmembrane Conductance Regulator Genetic Techniques Glycosylation Humans Immunologic Techniques Journal Article Research Support, Non-U.S. Gov't

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Carlos M Farinha, Deborah Penque, Mónica Roxo-Rosa, Gergely Lukacs, Robert Dormer, Margaret McPherson, Malcolm Pereira, Alice G M Bot, Huub Jorna, Rob Willemsen, Hugo Dejonge, Ghanshyam D Heda, Christopher R Marino, Pascale Fanen, Alexandre Hinzpeter, Joanna Lipecka, Janine Fritsch, Martina Gentzsch, Aleksander Edelman, Margarida D Amaral. 2004. Biochemical methods to assess CFTR expression and membrane localization.. https://doi.org/10.1016/j.jcf.2004.05.017

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