Microelectrodes and their use to assess ion channel function.

Aleksander Edelman; Marc Chanson; Martin J Hug

doi:10.1016/j.jcf.2004.05.024

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Microelectrodes and their use to assess ion channel function.

Aleksander Edelman·Marc Chanson·Martin J Hug

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Abstract

Impalement of living cells with microelectrodes (MEs) is a useful approach to measure a variety of biological parameters such as membrane potential (Vm), intracellular free ion concentrations and cell-to-cell communication. Despite such widespread applications, the number of trained researchers capable of using MEs is diminishing. In this article, we describe the fundamentals of the preparation and the proper use of MEs to study the physiology of transepithelial ion transport.

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Animals Biological Transport Epithelial Cells Fluorescent Dyes Ion Channel Gating Ion Channels Ion Transport Microelectrodes Journal Article Research Support, Non-U.S. Gov't Review

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Aleksander Edelman, Marc Chanson, Martin J Hug. 2004. Microelectrodes and their use to assess ion channel function.. https://doi.org/10.1016/j.jcf.2004.05.024

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