Chromatic immunoassay based on polydiacetylene vesicles.

Yan-Lei Su; Jin-Ru Li; Long Jiang

doi:10.1016/j.colsurfb.2004.08.010

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Chromatic immunoassay based on polydiacetylene vesicles.

Yan-Lei Su·Jin-Ru Li·Long Jiang

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Abstract

A new approach of chromatic immunoassay based on polydiacetylene vesicles is described. Antibodies were covalently coupled with mixed vesicles of 10,12-tricosadiynoic acid (TCDA) and dimyristoylphosphatidycholine (DMPC). The vesicle-antibody conjugates were irradiated with UV light to yield a blue-colored polydiacetylene. After antigen injection, specific immunoreactions took place at the vesicle surface alter polydiacetylene conformation and lead to a color change from blue to red. The chromatic immunoassay described here is simple, rapid, sensitive; the color change was readily discernible by naked eye when the concentration of antigen is 1 ng/mL. Incorporation of DMPC in the mixed vesicles increases the sensitivity of the chromatic immunoassay.

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Acetylene Antibodies Colorimetry Dimyristoylphosphatidylcholine Fatty Acids, Unsaturated Immunoassay Particle Size Polyacetylene Polymer Polymers Polyynes Sensitivity and Specificity Surface Properties Journal Article Research Support, Non-U.S. Gov't

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Yan-Lei Su, Jin-Ru Li, Long Jiang. 2004-10-10. Chromatic immunoassay based on polydiacetylene vesicles.. https://doi.org/10.1016/j.colsurfb.2004.08.010

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