Dynamic ion channel activation scheduling in patch clamp on a chip.

Vikram Krishnamurthy

doi:10.1109/tnb.2004.833681

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Dynamic ion channel activation scheduling in patch clamp on a chip.

Vikram Krishnamurthy

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Abstract

In 2002, Fertig et al. made a remarkable invention: the first successful demonstration of a patch clamp on a chip--a planar quartz-based biological chip that contains up to several hundred ion channels. This patch-clamp chip can be used in massively parallel screens for ion channel activity, thereby providing a high-throughput screening tool for drug discovery efforts. In this paper, we propose computationally efficient dynamic stochastic scheduling algorithms for activating individual ion channels in the patch-clamp chip. By formulating the ion channel activation scheduling problem as a partially observed Markov decision process with a multiarmed bandit structure, near-optimal dynamic scheduling for activation of the individual channels is achieved to optimize the information gained from the patch-clamp chip. Numerical examples using state-of-the-art algorithms developed recently in artificial intelligence and operations research are presented to illustrate these dynamic ion channel (macromolecule) activation scheduling algorithms.

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Algorithms Artificial Intelligence Computer Simulation Decision Support Techniques Ion Channel Gating Ion Channels Markov Chains Microchip Analytical Procedures Models, Biological Models, Statistical Patch-Clamp Techniques Evaluation Study Journal Article Research Support, Non-U.S. Gov't

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Vikram Krishnamurthy. 2004. Dynamic ion channel activation scheduling in patch clamp on a chip.. https://doi.org/10.1109/tnb.2004.833681

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