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PubMed · 15474076

Normal expression of isoforms activating cyclic adenosine monophosphate responsive element modulator in patients with spermatid maturation arrest.

Irina Palermo·Giuseppe Arcidiacono·Nunziata Barone·Fabrizio Italia·Mario Arizzi·Giovanni Spera·Giovanni Bartoloni·Silvana Di Mercurio·Enzo Vicari·Aldo E Calogero
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Abstract

OBJECTIVE: To evaluate whether defective cyclic adenosine monophosphate responsive element modulator (CREM) expression is the causative factor of spermatid maturation arrest (SMA). DESIGN: Comparative evaluation of the testicular histology in patients with SMA or normal spermatogenesis. SETTING: University clinic of andrology. PATIENT(S): Azoospermic patients undergoing testicular biopsy. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Expression of CREMtau in quantitative immunohistochemistry analysis of testicular biopsy samples. RESULT(S): Regular CREM expression was observed in the tubules with round, but not elongated, spermatids of patients with SMA (n = 9). Quantitative analysis showed that round spermatids of patients with SMA had a staining intensity similar to that observed in controls (n = 7). CONCLUSION(S): Lack of spermatid elongation was not due to defective CREM expression. Therefore, CREM did not play a pathogenetic role in the onset of SMA in humans.

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BiopsyCellular SenescenceCyclic AMP Response Element ModulatorDNA-Binding ProteinsHumansImmunohistochemistryMaleOligospermiaRepressor ProteinsSpermatidsTestisJournal Article

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Irina Palermo, Giuseppe Arcidiacono, Nunziata Barone, Fabrizio Italia, Mario Arizzi, Giovanni Spera, Giovanni Bartoloni, Silvana Di Mercurio, Enzo Vicari, Aldo E Calogero. 2004. Normal expression of isoforms activating cyclic adenosine monophosphate responsive element modulator in patients with spermatid maturation arrest.. https://doi.org/10.1016/j.fertnstert.2004.05.077

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