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Plasma cell ontogeny defined by quantitative changes in blimp-1 expression.

Axel Kallies·Jhagvaral Hasbold·David M Tarlinton·Wendy Dietrich·Lynn M Corcoran·Philip D Hodgkin·Stephen L Nutt
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Abstract

Plasma cells comprise a population of terminally differentiated B cells that are dependent on the transcriptional regulator B lymphocyte--induced maturation protein 1 (Blimp-1) for their development. We have introduced a gfp reporter into the Blimp-1 locus and shown that heterozygous mice express the green fluorescent protein in all antibody-secreting cells (ASCs) in vivo and in vitro. In vitro, these cells display considerable heterogeneity in surface phenotype, immunoglobulin secretion rate, and Blimp-1 expression levels. Importantly, analysis of in vivo ASCs induced by immunization reveals a developmental pathway in which increasing levels of Blimp-1 expression define developmental stages of plasma cell differentiation that have many phenotypic and molecular correlates. Thus, maturation from transient plasmablast to long-lived ASCs in bone marrow is predicated on quantitative increases in Blimp-1 expression.

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AnimalsAntibody-Producing CellsCell DifferentiationGene Expression RegulationMiceMice, Inbred BALB CMice, Inbred C57BLPhenotypePlasma CellsPositive Regulatory Domain I-Binding Factor 1Repressor ProteinsTranscription FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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Axel Kallies, Jhagvaral Hasbold, David M Tarlinton, Wendy Dietrich, Lynn M Corcoran, Philip D Hodgkin, Stephen L Nutt. 2004-10-18. Plasma cell ontogeny defined by quantitative changes in blimp-1 expression.. https://doi.org/10.1084/jem.20040973

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