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Common double- and single-stranded DNA binding factor for a sterol regulatory element.

H C Stark·O Weinberger·J Weinberger
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Abstract

A cis-acting element necessary for sterol regulation, SRE-1, has previously been identified in the promoters of the low density lipoprotein receptor, hydroxymethylglutaryl (HMG)-CoA reductase, and HMG-CoA synthase genes. In this report we describe a nuclear factor, SRE-BF, isolated from Chinese hamster ovary nuclear extracts, that binds to the SRE-1 octanucleotide sequence. In addition to sequence-specific binding to SRE-1, as indicated by competition analysis with double-stranded DNA fragments, single-stranded oligomer DNA sequences also compete for binding in a sequence-specific fashion. Photochemical cross-linking experiments suggest that a common protein factor, with apparent molecular mass of 45-49 kDa, recognizes both single-stranded and double-stranded SRE-1. The binding specificity of SRE-BF to single-stranded SRE-1 closely correlates with the reported in vivo ability of SRE-1 to direct sterol responsiveness of transcription.

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AnimalsBase SequenceBinding, CompetitiveCHO CellsCell NucleusCricetinaeDNA ProbesDNA-Binding ProteinsKineticsMolecular Sequence DataOligodeoxyribonucleotidesOligonucleotide ProbesPolymerase Chain ReactionRegulatory Sequences, Nucleic AcidRestriction MappingSterolsTranscription, GeneticUltraviolet RaysJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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H C Stark, O Weinberger, J Weinberger. 1992-03-15. Common double- and single-stranded DNA binding factor for a sterol regulatory element.. https://doi.org/10.1073/pnas.89.6.2180

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