Epidermal cell analysis by RT-PCR.

Tammy-Claire Troy; Robert Man-Kit Cheung; Kursad Turksen

doi:10.1385/1-59259-830-7:121

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Epidermal cell analysis by RT-PCR.

Tammy-Claire Troy·Robert Man-Kit Cheung·Kursad Turksen

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Abstract

Reverse transcription polymerase chain reaction is used for the semiquantitative analysis of epidermal gene expression, particularly when immunolocalization is not feasible because of the lack of antibodies available for novel genes. This chapter is therefore devoted to the delineation of a reliable reverse transcription polymerase chain reaction method to analyze gene expression both in vivo and in vitro.

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Animals Cells, Cultured Epidermal Cells Epidermis Epithelial Cells Mice RNA, Messenger Reverse Transcriptase Polymerase Chain Reaction Journal Article Research Support, Non-U.S. Gov't

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Tammy-Claire Troy, Robert Man-Kit Cheung, Kursad Turksen. 2005. Epidermal cell analysis by RT-PCR.. https://doi.org/10.1385/1-59259-830-7%3A121

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