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Analysis of tissue-specific DNA methylation during development.

Jun Ohgane·Naka Hattori·Kunio Shiota
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Abstract

The establishment of a cell identification method more accurate than the conventional morphological and cell-type-specific marker analyses has been desired. DNA methylation is related to the gene activity including gene-silencing and is a key mechanism of epigenetics underling cellular differentiaion and development in mammals. Recent studies indicated that there exist unique genomic DNA methylation profiles specific to the cell type. DNA methylation profiles a mechanism for memorizing the set of genes inherent in individual type of cells. In this chapter, we present the methods to analyze DNA methylation status for identifying cells or tissues.

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AnimalsAutoradiographyBlotting, SouthernCells, CulturedCpG IslandsDNA MethylationGene Expression RegulationGenomic ImprintingGrowth and DevelopmentHumansMaleSpermatozoaJournal Article

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Jun Ohgane, Naka Hattori, Kunio Shiota. 2005. Analysis of tissue-specific DNA methylation during development.. https://doi.org/10.1385/1-59259-830-7%3A371

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