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PubMed · 15509804

Regulation of cell cycle-specific gene expression through cyclin-dependent kinase-mediated phosphorylation of the forkhead transcription factor Fkh2p.

Aline Pic-Taylor·Zoulfia Darieva·Brian A Morgan·Andrew D Sharrocks
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Abstract

The forkhead transcription factor Fkh2p acts in a DNA-bound complex with Mcm1p and the coactivator Ndd1p to regulate cell cycle-dependent expression of the CLB2 gene cluster in Saccharomyces cerevisiae. Here, we demonstrate that Fkh2p is a target of cyclin-dependent protein kinases and that phosphorylation of Fkh2p promotes interactions between Fkh2p and the coactivator Ndd1p. These phosphorylation-dependent changes in the Fkh2p-Ndd1p complex play an important role in the cell cycle-regulated expression of the CLB2 cluster. Our data therefore identify an important regulatory target for cyclin-dependent kinases in the cell cycle and further our molecular understanding of the key cell cycle regulatory transcription factor Fkh2p.

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Binding SitesCDC28 Protein Kinase, S cerevisiaeCell CycleCell Cycle ProteinsCyclin BCyclin-Dependent KinasesForkhead Transcription FactorsGene Expression Regulation, FungalGenes, FungalMacromolecular SubstancesModels, BiologicalMultigene FamilyMutagenesisPhosphorylationRecombinant Fusion ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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Aline Pic-Taylor, Zoulfia Darieva, Brian A Morgan, Andrew D Sharrocks. 2004. Regulation of cell cycle-specific gene expression through cyclin-dependent kinase-mediated phosphorylation of the forkhead transcription factor Fkh2p.. https://doi.org/10.1128/mcb.24.22.10036-10046.2004

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