Neuroendocrine responses mediate macrophage function after trauma.

Sirish Maddali; Philip P Stapleton; Tracy A Freeman; Gordon P Smyth; Michael Duff; Zhaoping Yan; John M Daly

doi:10.1016/j.surg.2004.03.001

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Neuroendocrine responses mediate macrophage function after trauma.

Sirish Maddali·Philip P Stapleton·Tracy A Freeman·Gordon P Smyth·Michael Duff·Zhaoping Yan·John M Daly

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Abstract

BACKGROUND: Clearly understanding the interactions between macrophage (M phi)-generated inflammatory mediators and the neuroendocrine system in regulating immune function after traumatic injury may aid in reversing trauma-mediated immune dysfunction and diminish the incidence and severity of infection in the traumatized patient. METHODS: Trauma consisted of an open femur fracture and 40% retro-orbital hemorrhage (Trauma) or anesthesia alone (Control). Female Balb/C mice (6-8 weeks) with intact adrenal glands (Intact) or a bilateral adrenalectomy (ADX) were used. For glucocorticoid studies, corticosterone or a vehicle was administered via intraperitoneal (ip) injection 2 hours before the trauma. Splenic M phis were harvested and prostaglandin E(2) (PGE(2)) and interleukin-6 (IL-6) production, and mRNA, cyclooxygenase-2 (COX-2) protein, and nuclear factor kappa B (NF-kappa B) activity were measured. RESULTS: M phi, PGE(2) and IL-6 production in Trauma+Intact mice was significantly increased compared with Control+Intact mice. Adrenalectomy decreased these levels to Control levels. Similar changes were observed for COX-2 and IL-6 expression. M phi nuclear NF-kappa B levels were increased in Trauma+Intact mice compared with controls. Adrenalectomy abrogated this increase. Treating Trauma+Intact mice with RU-486 did not restore PGE(2) and IL-6 production or COX-2 and IL-6 messenger RNA to control levels. Administering exogenous glucocorticoid to Intact mice did not increase PGE(2) and IL-6 production or COX-2 and IL-6 mRNA to Trauma levels. CONCLUSIONS: The neuroendocrine system upregulates certain M phi inflammatory mediators, including PGE(2), IL-6, and NF-kappa B, after trauma. This upregulation does not seem to be mediated via glucocorticoids and possibly may be mediated via catecholamines. Elucidation of the interactions between the neuroendocrine system, the immune system, and inflammatory mediator secretion might provide novel therapeutic strategies for the injured patient.

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Animals Corticosterone Cyclooxygenase 2 Disease Models, Animal Female Femoral Fractures Macrophages Mice Mice, Inbred BALB C Mifepristone Neurosecretory Systems Prostaglandin-Endoperoxide Synthases RNA, Messenger Reverse Transcriptase Polymerase Chain Reaction Spleen Wounds and Injuries Journal Article Research Support, U.S. Gov't, P.H.S.

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Sirish Maddali, Philip P Stapleton, Tracy A Freeman, Gordon P Smyth, Michael Duff, Zhaoping Yan, John M Daly. 2004. Neuroendocrine responses mediate macrophage function after trauma.. https://doi.org/10.1016/j.surg.2004.03.001

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