Relationship between local structural entropy and protein thermostability.

Chen-Hsiung Chan; Han-Kuen Liang; Nai-Wan Hsiao; Ming-Tat Ko; Ping-Chiang Lyu; Jenn-Kang Hwang

doi:10.1002/prot.20263

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Relationship between local structural entropy and protein thermostability.

Chen-Hsiung Chan·Han-Kuen Liang·Nai-Wan Hsiao·Ming-Tat Ko·Ping-Chiang Lyu·Jenn-Kang Hwang

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Abstract

We developed a technique to compute structural entropy directly from protein sequences. We explored the possibility of using structural entropy to identify residues involved in thermal stabilization of various protein families. Examples include methanococcal adenylate kinase, Ribonuclease HI and holocytochrome c(551). Our results show that the positions of the largest structural entropy differences between wild type and mutant usually coincide with the residues relevant to thermostability. We also observed a good linear relationship between the average structural entropy and the melting temperatures for adenylate kinase and its chimeric constructs. To validate this linear relationship, we compiled a large dataset comprised of 1153 sequences and found that most protein families still display similar linear relationships. Our results suggest that the multitude of interactions involved in thermal stabilization may be generalized into the tendency of proteins to maintain local structural conservation. The linear relationship between structural entropy and protein thermostability should be useful in the study of protein thermal stabilization.

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Adenylate Kinase Amino Acid Sequence Bacterial Proteins Cytochrome c Group Entropy Enzyme Stability Models, Molecular Molecular Sequence Data Mutation Protein Structure, Tertiary Proteins Ribonuclease H Transition Temperature Journal Article Research Support, Non-U.S. Gov't

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Chen-Hsiung Chan, Han-Kuen Liang, Nai-Wan Hsiao, Ming-Tat Ko, Ping-Chiang Lyu, Jenn-Kang Hwang. 2004-12-01. Relationship between local structural entropy and protein thermostability.. https://doi.org/10.1002/prot.20263

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