Evidence for two cDNA clones encoding human GM2-activator protein.

S Nagarajan; H C Chen; S C Li; Y T Li; J M Lockyer

doi:10.1042/bj2820807

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Evidence for two cDNA clones encoding human GM2-activator protein.

S Nagarajan·H C Chen·S C Li·Y T Li·J M Lockyer

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Abstract

Two cDNAs encoding GM2 activator, pGM2A (648 bp) and GAP (1093 bp), were isolated from human placenta lambda gt11 libraries. The DNA sequence of pGM2A from 1 to 302 was almost identical with GAP, but diverged from 303-648. PCR was used to demonstrate the presence of both species of GM2 activator in placental RNA. Both cDNAs hybridized to mRNAs of approximately 2.3 kb and to identical single bands on genomic Southern blots.

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Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular DNA Fibroblasts G(M2) Activator Protein Gene Amplification Genome, Human Humans Molecular Sequence Data Peptide Fragments Placenta Polymerase Chain Reaction Proteins RNA, Messenger Journal Article Research Support, U.S. Gov't, P.H.S.

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S Nagarajan, H C Chen, S C Li, Y T Li, J M Lockyer. 1992-03-15. Evidence for two cDNA clones encoding human GM2-activator protein.. https://doi.org/10.1042/bj2820807

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