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Developing a peptide-based near-infrared molecular probe for protease sensing.

Wellington Pham·Yongdoo Choi·Ralph Weissleder·Ching-Hsuan Tung
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Abstract

Recently near-infrared (NIR) molecular probes have become important reporter molecules for a number of types of in vivo biomedical imaging. A peptide-based NIR fluorescence probe consisting of a NIR fluorescence emitter (Cy5.5), a NIR fluorescence absorber (NIRQ820), and a protease selective peptide sequence was designed to sense protease activity. Using a MMP-7 model, we showed that NIRQ820 efficiently absorbs the emission energy of Cy5.5 resulting in a low initial signal. Upon reacting with its target, MMP-7, the fluorescence signal of the designed probe was increased by 7-fold with a K(cat)/K(m) of 100 000 M(-)(1) s(-)(1). The described synthetic strategy should have wide application for other NIR probe preparations.

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AnimalsCell Line, TumorHumansMatrix Metalloproteinase 7MetalloendopeptidasesMiceMice, NudeMolecular ProbesPeptide FragmentsPeptide HydrolasesSpectroscopy, Near-InfraredJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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Wellington Pham, Yongdoo Choi, Ralph Weissleder, Ching-Hsuan Tung. Developing a peptide-based near-infrared molecular probe for protease sensing.. https://doi.org/10.1021/bc049924s

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