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PubMed · 15557804

Optimization of 13C direct detection NMR methods.

Nobuhisa Shimba·Helena Kovacs·Alan S Stern·Anson M Nomura·Ichio Shimada·Jeffrey C Hoch·Charles S Craik·Volker Dötsch
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Abstract

(13)C-detected experiments are still limited by their inherently lower sensitivity, as compared to the equivalent (1)H-detected experiments. Improving the sensitivity of (13)C detection methods remains a significant area of NMR research that may provide better means for studying large macromolecular systems by NMR. In this communication, we show that (13)C-detected experiments are less sensitive to the salt concentration of the sample solution than (1)H-detected experiments. In addition, acquisition can be started with anti-phase coherence, resulting in higher sensitivity due to the elimination of the final INEPT transfer step.

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Carbon IsotopesLeucineMagnetic Resonance SpectroscopySensitivity and SpecificitySodium ChlorideSolutionsJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Nobuhisa Shimba, Helena Kovacs, Alan S Stern, Anson M Nomura, Ichio Shimada, Jeffrey C Hoch, Charles S Craik, Volker Dötsch. 2004. Optimization of 13C direct detection NMR methods.. https://doi.org/10.1023/b%3Ajnmr.0000048855.35771.11

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