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PubMed · 15568701

Y-chromosome STR system, Y-PLEX 12, for forensic casework: development and validation.

Jaiprakash G Shewale·Huma Nasir·Elaine Schneida·Ann Marie Gross·Bruce Budowle·Sudhir K Sinha
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Abstract

The Y-PLEX 12 system, developed for use in human identification, enables simultaneous amplification of eleven polymorphic short tandem repeat (STR) loci, namely DYS392, DYS390, DYS385 a/b, DYS393, DYS389I, DYS391, DYS389II, DYS 19, DYS439 and DYS438, residing on the Y chromosome and Amelogenin. Amelogenin provides results for gender identification and serves as internal control for PCR. The validation studies were performed according to the DNA Advisory Board's (DAB) Quality Assurance Standards. The minimal sensitivity of the Y-PLEX 12 system was 0.1 ng of male DNA. The mean stutter values ranged between 3.76-15.72%. A full male profile was observed in mixture samples containing 0.5 ng of male DNA and up to 400 ng of female DNA. Amelogenin did not adversely affect the amplification of Y-STRs in mixture samples containing male and female DNA. The primers for the Y-STR loci present in Y-PLEX 12 are specific for human DNA and some higher primates. None of the primate samples tested provided a complete profile at all 11 Y-STR loci amplified with the Y-PLEX 12 system. Y-PLEX 12 is a sensitive, valid, reliable, and robust multiplex system for forensic analysis, and it can be used in human forensic and male lineage identification cases.

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AmelogeninAnimalsChromosomes, Human, YDNA FingerprintingDNA PrimersDental Enamel ProteinsFemaleHumansMalePolymerase Chain ReactionPolymorphism, GeneticSequence Analysis, DNASex Determination ProcessesTandem Repeat SequencesTooth GermJournal ArticleValidation Study

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Jaiprakash G Shewale, Huma Nasir, Elaine Schneida, Ann Marie Gross, Bruce Budowle, Sudhir K Sinha. 2004. Y-chromosome STR system, Y-PLEX 12, for forensic casework: development and validation.. https://pubmed.ncbi.nlm.nih.gov/15568701/

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