RNA interference: past, present and future.

Tessa N Campbell; Francis Y M Choy

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PubMed · 15580776

RNA interference: past, present and future.

Tessa N Campbell·Francis Y M Choy

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Abstract

RNA interference (RNAi) is the sequence-specific gene silencing induced by double-stranded RNA. RNAi is mediated by 21-23 nucleotide small interfering RNAs (siRNAs) which are produced from long double-stranded RNAs by RNAse II-like enzyme Dicer. The resulting siRNAs are incorporated into a RNA-induced silencing complex (RISC) that targets and cleaves mRNA complementary to the siRNAs. Since its inception in 1998, RNAi has been demonstrated in organisms ranging from trypanosomes to nematodes to vertebrates. Potential uses already in progress include the examination of specific gene function in living systems, the development of anti-viral and anti-cancer therapies, and genome-wide screens. In this review, we discuss the landmark discoveries that established the contextual framework leading up to our current understanding of RNAi. We also provide an overview of current developments and future applications.

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Animals Communicable Diseases Forecasting Genes, Dominant Genetic Diseases, Inborn Humans Molecular Biology Neoplasms RNA Interference RNA, Small Interfering Journal Article Research Support, Non-U.S. Gov't Review

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Tessa N Campbell, Francis Y M Choy. 2005. RNA interference: past, present and future.. https://pubmed.ncbi.nlm.nih.gov/15580776/

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