[A new in vitro model to quantify angiogenesis].

Mahtab Bahramsoltani; Johanna Plendl

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PubMed · 15586254

[A new in vitro model to quantify angiogenesis].

Mahtab Bahramsoltani·Johanna Plendl

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Abstract

At present the therapeutical use of proangiogenic factors is in the focus of interest. Quantitation of angiogenesis, which is done particularly in animal models, is a prerequisite for the determination of the angiogenic effect of a particular substance. In the frame of this study a new method for quantitation of angiogenesis in vitro was established. Endothelial cells were incubated with angiogenic factors to be tested, and documented photographically in defined microscopical sectors over a certain time period. Subsequently defined stages of angiogenesis (1-8) were assigned. For each sector all stages assigned were summarized and compared with each other. This and additional morphometric studies showed that angiogenesis up to the development of lumenised capillary-like structures in vitro can be quantified. Validation of the method showed that routine and reproducible accomplishment should be possible for different investigators with a maintainable effort of time and costs.

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Angiogenesis Inducing Agents Animal Testing Alternatives Animals Cells, Cultured Endothelial Cells Humans Models, Animal Neovascularization, Physiologic English Abstract Journal Article Review

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Mahtab Bahramsoltani, Johanna Plendl. 2004. [A new in vitro model to quantify angiogenesis].. https://pubmed.ncbi.nlm.nih.gov/15586254/

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