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PubMed · 15597042

Neuronal tau induces DNA conformational changes observed by atomic force microscopy.

Mei-hua Qu·Hui Li·Rui Tian·Chun-lai Nie·Ying Liu·Bao-shan Han·Rong-qiao He
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Abstract

Neuronal tau associates with chromosome scaffold and localizes in the nuclear and the nucleolar organization regions in neuronal and some non-neuronal cells. Observing the interaction of neuronal tau with DNA under AFM shows that tau binds to DNA as a monomer, and tau-DNA complex forms a beads-on-a-string structure when the mass ratio is 1:10 (molar ratio of tau/DNA approximately 1:700 bp). A beads-on-a-coil structure, in which tau is as polymers, will appear when the mass ratio is up to 1:5 (molar ratio of tau/DNA approximately 1:350 bp). The present observation that neuronal tau bends the DNA double strands indicates that the appearance of the tau-DNA complex is dependent upon the mass (or molar) ratio of tau and DNA.

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DNAHumansMicroscopy, Atomic ForceNucleic Acid ConformationProtein Bindingtau ProteinsComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Mei-hua Qu, Hui Li, Rui Tian, Chun-lai Nie, Ying Liu, Bao-shan Han, Rong-qiao He. 2004-12-22. Neuronal tau induces DNA conformational changes observed by atomic force microscopy.. https://pubmed.ncbi.nlm.nih.gov/15597042/

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