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PubMed · 15603260

Engineering protein A affinity chromatography.

Alois Jungbauer·Rainer Hahn
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Abstract

Staphylococcal protein A can selectively interact with immunoglobulins. This protein is widely used as a ligand for affinity chromatography to purify therapeutic antibodies on an industrial scale. This type of affinity chromatography constitutes a generic step in processing antibodies. Questions of scale-up, design of chromatographic conditions, clearance of adventitious agents and operational modes, such as continuous operation or purification of antibodies in expanded-bed mode, will be addressed in this review.

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Antibodies, MonoclonalChromatography, AffinityImmunoglobulin GLigandsModels, ChemicalProtein EngineeringStaphylococcal Protein AJournal Article

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Alois Jungbauer, Rainer Hahn. 2004. Engineering protein A affinity chromatography.. https://pubmed.ncbi.nlm.nih.gov/15603260/

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