Global analysis of protein function using protein microarrays.

Michael G Smith; Ghil Jona; Jason Ptacek; Geeta Devgan; Heng Zhu; Xiaowei Zhu; Michael Snyder

doi:10.1016/j.mad.2004.09.019

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Global analysis of protein function using protein microarrays.

Michael G Smith·Ghil Jona·Jason Ptacek·Geeta Devgan·Heng Zhu·Xiaowei Zhu·Michael Snyder

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Abstract

Protein microarrays containing thousands of proteins arrayed at high density can be prepared and probed for a wide variety of activities, thereby allowing the large scale analysis of many proteins simultaneously. In addition to identifying the activities of many previously uncharacterized proteins, protein microarrays can reveal new activities of well-characterized proteins, thus providing new insights about the functions of these proteins. Below, we describe the construction and use of protein microarrays and their applications using yeast as a model system.

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Animals Humans Protein Array Analysis Proteome Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Journal Article Review

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Michael G Smith, Ghil Jona, Jason Ptacek, Geeta Devgan, Heng Zhu, Xiaowei Zhu, Michael Snyder. 2005. Global analysis of protein function using protein microarrays.. https://doi.org/10.1016/j.mad.2004.09.019

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