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PubMed · 15611091

Physical and functional interactions between STAP-2/BKS and STAT5.

Yuichi Sekine·Tetsuya Yamamoto·Taro Yumioka·Kenji Sugiyama·Satoshi Tsuji·Kenji Oritani·Kazuya Shimoda·Mayu Minoguchi·Akihiko Yoshimura·Tadashi Matsuda
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Abstract

Signal-transducing adaptor protein family of proteins (STAPs), which currently contains two members, are proposed to be adaptor molecules because of their pleckstrin homology (PH) and Src-homology 2 (SH2)-like domains. STAP-1 has been shown to interact with STAT5 and the tyrosine kinase Tec. With regard to STAP-2/BKS functions, immunoprecipitation experiments and intracellular stainings revealed STAP-2/BKS binds STAT5 in several types of cells. Mutational studies revealed that the PH- and SH2-like domains of STAP-2/BKS interacted with the C-terminal region of STAT5. STAP-2/BKS and STAT5 were found to constitutively co-localize in the cytoplasm of resting cells, but STAP-2/BKS was found to dissociate upon STAT5 phosphorylation, suggesting a role in regulating signaling of STAT5. The physiological role of these interactions is not fully understood, but in studies of overexpression of STAP-2/BKS, cytokine-induced tyrosine phosphorylation and transcriptional activation of STAT5 was diminished. In addition, thymocytes from STAP-2/BKS-deficient mice showed the enhanced interleukin-2-dependent cell growth. Taken together, STAP-2/BKS is an additional modulator of STAT5-mediated signaling.

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Adaptor Proteins, Signal TransducingAnimalsCOS CellsCell LineCytoplasmDNA-Binding ProteinsErythropoietinFluorescent Antibody Technique, IndirectGlutathione TransferaseHeLa CellsHumansImmunoblottingImmunoprecipitationInterleukin-2Interleukin-3MiceMice, KnockoutMilk ProteinsPhosphoproteinsPhosphorylationProtein BindingProtein Structure, TertiarySTAT5 Transcription FactorSignal TransductionThymus GlandTime FactorsTrans-ActivatorsTranscriptional ActivationTransfectionTumor Suppressor ProteinsTyrosineJournal ArticleResearch Support, Non-U.S. Gov't

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Yuichi Sekine, Tetsuya Yamamoto, Taro Yumioka, Kenji Sugiyama, Satoshi Tsuji, Kenji Oritani, Kazuya Shimoda, Mayu Minoguchi, Akihiko Yoshimura, Tadashi Matsuda. 2004-12-17. Physical and functional interactions between STAP-2/BKS and STAT5.. https://doi.org/10.1074/jbc.m411692200

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