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PubMed · 15621664

Solving a sequencing problem in the vertebrate mitochondrial control region using phylogenetic comparisons.

Julie Feinstein·Joel Cracraft
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Abstract

The mitochondrial control region (mtCR) of the bird-of-paradise, Phonygammus keraudrenii, the Trumpet Manucode, contains a unique arrangement of homopolymers and short tandem repeats. Homopolymers occur within a few hundred bases of each other, trapping sequence information between unsequenceable barriers. A comparative strategy, involving other manucode species, allowed the prediction of primer sites in the inaccessible region. The method is suggested for similar sequencing problems.

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AnimalsBase SequenceDNA PrimersDNA, MitochondrialLocus Control RegionMolecular Sequence DataPasseriformesPhylogenyRepetitive Sequences, Nucleic AcidSequence AlignmentSequence Analysis, DNAComparative StudyJournal Article

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Julie Feinstein, Joel Cracraft. Solving a sequencing problem in the vertebrate mitochondrial control region using phylogenetic comparisons.. https://doi.org/10.1080/10425170400012909

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