Microarray-based resequencing of multiple Bacillus anthracis isolates.

Michael E Zwick; Farrell Mcafee; David J Cutler; Timothy D Read; Jacques Ravel; Gregory R Bowman; Darrell R Galloway; Alfred Mateczun

doi:10.1186/gb-2004-6-1-r10

PubMed · 15642093

Microarray-based resequencing of multiple Bacillus anthracis isolates.

Michael E Zwick·Farrell Mcafee·David J Cutler·Timothy D Read·Jacques Ravel·Gregory R Bowman·Darrell R Galloway·Alfred Mateczun

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Abstract

We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 x 10(-7)) and by comparison to independently generated shotgun sequence (discrepancy rate < 2.5 x 10(-6)). Population genomics studies of microbial pathogens using rapid resequencing technologies such as resequencing arrays are critical for recognizing newly emerging or genetically engineered strains.

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Bacillus anthracis Chromosomes, Bacterial Genetic Variation Genome, Bacterial Molecular Sequence Data Oligonucleotide Array Sequence Analysis Recombination, Genetic Reproducibility of Results Sequence Analysis, DNA Time Factors Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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Michael E Zwick, Farrell Mcafee, David J Cutler, Timothy D Read, Jacques Ravel, Gregory R Bowman, Darrell R Galloway, Alfred Mateczun. 2004-12-17. Microarray-based resequencing of multiple Bacillus anthracis isolates.. https://doi.org/10.1186/gb-2004-6-1-r10

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