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A selective experiment for the sequential protein backbone assignment from 3D heteronuclear spectra.

Wolfgang Bermel·Ivano Bertini·Isabella C Felli·Roberta Pierattelli·Paul R Vasos
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Abstract

Two modifications of the triple-resonance CANCO sequence, designed for backbone assignment in proteins [Angew. Chem. Int. Ed. 43 (2004) 2257], are presented here. These two new sequences display the intra-residue Ca-CO correlation selectively, while in the original sequence both the inter- and the intra-residue correlations were present. In addition, one of the two variants benefits from an improved sensitivity. Both sequences are a useful complement to the CANCO sequence for facile sequence-specific protein assignment by protonless NMR.

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Calcium-Binding ProteinsCarbon IsotopesNitrogen IsotopesNuclear Magnetic Resonance, BiomolecularJournal ArticleResearch Support, Non-U.S. Gov't

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Wolfgang Bermel, Ivano Bertini, Isabella C Felli, Roberta Pierattelli, Paul R Vasos. 2005. A selective experiment for the sequential protein backbone assignment from 3D heteronuclear spectra.. https://doi.org/10.1016/j.jmr.2004.11.005

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