Conditional UAS-targeted repression in Drosophila.

Alberto Pascual; Kai-Lian Huang; Thomas Preat

doi:10.1093/nar/gni006

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Conditional UAS-targeted repression in Drosophila.

Alberto Pascual·Kai-Lian Huang·Thomas Preat

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Abstract

The Gal4-UAS enhancer trap system is useful for driving gene expression in various tissues. A new tool that extends Gal4 technology is described here. A fusion protein containing the Gal4 binding domain and the repression domain of the isolator suppressor of hairy wing was placed under the control of a heat shock-inducible promoter. The construct mediates the conditional repression of genes located downstream of a UAS sequence. The repressive effects of the chimeric protein on fasII gene expression were tested by western-blot analysis and in brain sections of adult Drosophila. Owing to the increasing number of Gal4 and UAS transgenic lines, this versatile system will facilitate the study of gene function.

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Animals DNA-Binding Proteins Drosophila Drosophila Proteins Gene Expression Regulation Gene Silencing Hot Temperature Mutagenesis, Insertional Nuclear Proteins Protein Structure, Tertiary Recombinant Fusion Proteins Regulatory Sequences, Nucleic Acid Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors Journal Article Research Support, Non-U.S. Gov't

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Alberto Pascual, Kai-Lian Huang, Thomas Preat. 2005-01-13. Conditional UAS-targeted repression in Drosophila.. https://doi.org/10.1093/nar%2Fgni006

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