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An improved method for proteomics studies in C. elegans by fluorogenic derivatization, HPLC isolation, enzymatic digestion and liquid chromatography--tandem mass spectrometric identification.

Mayumi Masuda·Hiroshi Saimaru·Norio Takamura·Kazuhiro Imai
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Abstract

An improved method for proteomics studies, which includes the fluorogenic derivertization of protein mixtures with 7-chloro-4-(dimethylaminoethylaminosulfonyl)-2,1,3-benzoxadiazole (DAABD-Cl), followed by HPLC isolation, enzymatic digestion and identification of the derivatized proteins by HPLC-electrospray ionization (ESI)-MS/MS with the probability-based protein identification algorithm, identified 103 proteins in the soluble extract (10 microg protein) of Caenorhabditis elegans.

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AlgorithmsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsChromatography, High Pressure LiquidChromatography, LiquidFluorescent DyesOxadiazolesProteomicsSpectrometry, Mass, Electrospray IonizationSulfonamidesJournal Article

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Mayumi Masuda, Hiroshi Saimaru, Norio Takamura, Kazuhiro Imai. 2005. An improved method for proteomics studies in C. elegans by fluorogenic derivatization, HPLC isolation, enzymatic digestion and liquid chromatography--tandem mass spectrometric identification.. https://doi.org/10.1002/bmc.479

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