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PubMed · 15690981

cDNA microarray analysis in hepatocyte differentiation in Huh 7 cells.

Yo-ichi Yamashita·Mitsuo Shimada·Norifumi Harimoto·Shinji Tanaka·Ken Shirabe·Hiroyuki Ijima·Kohji Nakazawa·Junji Fukuda·Kazumori Funatsu·Yoshihiko Maehara
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Abstract

The risk of xenozoonosis infections poses the greatest obstacle against the clinical application of a hybrid artificial liver support system (HALSS). Primary human hepatocytes are an ideal source for HALSS, but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, we previously demonstrated the upregulation of hepatocyte-specific function by spheroid formation in polyurethane foam and by culturing with the histone deacetylase inhibitor, trichostatin A (TSA), in a human hepatoma cell line (Huh 7). In this article we analyze the gene expression profile using cDNA microarray (1281 genes) in spheroid formation or culturing with TSA in Huh 7 to determine the target genes in hepatocyte differentiation. In both the spheroid formation and in the culture with TSA, the Oct-3/4 transcription factor was upregulated more thantwofold, while the early growth response-1 (EGR-1) transactivator was downregulated less than 0.5-fold. These results indicate that expressions of Oct-3/4 and EGR-1 may be key factors in the induction of hepatocyte differentiation in Huh 7.

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Cell Culture TechniquesCell DifferentiationCell Line, TumorCell ProliferationDNA, ComplementaryDNA-Binding ProteinsDown-RegulationEarly Growth Response Protein 1Enzyme InhibitorsGene Expression ProfilingGene Expression RegulationHepatocytesHumansHydroxamic AcidsImmediate-Early ProteinsLiver, ArtificialOctamer Transcription Factor-3Oligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionSpheroids, CellularTranscription FactorsUp-RegulationJournal ArticleResearch Support, Non-U.S. Gov't

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Yo-ichi Yamashita, Mitsuo Shimada, Norifumi Harimoto, Shinji Tanaka, Ken Shirabe, Hiroyuki Ijima, Kohji Nakazawa, Junji Fukuda, Kazumori Funatsu, Yoshihiko Maehara. 2004. cDNA microarray analysis in hepatocyte differentiation in Huh 7 cells.. https://doi.org/10.3727/000000004783983396

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