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PubMed · 15691515

An enzyme-amplified diffraction-based immunoassay.

Richard W Loo·Pui L Tam·Jane Betty Goh·M Cynthia Goh
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Abstract

We have previously shown that a gold-conjugated secondary label can be used to reduce the limit of detection in a diffraction-based assay by more than 40-fold. We now show that by using a combination of a peroxidase-conjugated secondary label and a precipitating substrate the limit of detection in a diffraction-based assay can be reduced by more than 1000-fold. The response to secondary enhancement was linear for concentrations from 50 to 2000 pg/mL of antidigoxin.

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AnimalsCattleDigoxinImmunoenzyme TechniquesLasersPeroxidaseScattering, RadiationSensitivity and SpecificitySerum Albumin, BovineJournal ArticleResearch Support, Non-U.S. Gov't

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Richard W Loo, Pui L Tam, Jane Betty Goh, M Cynthia Goh. 2005-02-15. An enzyme-amplified diffraction-based immunoassay.. https://doi.org/10.1016/j.ab.2004.11.023

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