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PubMed · 15691978

Rapid and simple quantification of bacterial cells by using a microfluidic device.

Chieko Sakamoto·Nobuyasu Yamaguchi·Masao Nasu
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Abstract

This study investigated a microfluidic chip-based system (on-chip flow cytometry) for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count. The time required for this on-chip flow cytometry was only 30 min per 6 samples.

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Bacteriological TechniquesColony Count, MicrobialCulture MediaEscherichia coli O157Flow CytometryMicrofluidicsMicroscopy, FluorescenceOrganic ChemicalsPseudomonas putidaRiversStaining and LabelingTime FactorsComparative StudyEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Chieko Sakamoto, Nobuyasu Yamaguchi, Masao Nasu. 2005. Rapid and simple quantification of bacterial cells by using a microfluidic device.. https://doi.org/10.1128/aem.71.2.1117-1121.2005

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