Dynamic complex formation during the yeast cell cycle.

Ulrik de Lichtenberg; Lars Juhl Jensen; Søren Brunak; Peer Bork

doi:10.1126/science.1105103

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Dynamic complex formation during the yeast cell cycle.

Ulrik de Lichtenberg·Lars Juhl Jensen·Søren Brunak·Peer Bork

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Abstract

To analyze the dynamics of protein complexes during the yeast cell cycle, we integrated data on protein interactions and gene expression. The resulting time-dependent interaction network places both periodically and constitutively expressed proteins in a temporal cell cycle context, thereby revealing previously unknown components and modules. We discovered that most complexes consist of both periodically and constitutively expressed subunits, which suggests that the former control complex activity by a mechanism of just-in-time assembly. Consistent with this, we show that additional regulation through targeted degradation and phosphorylation by Cdc28p (Cdk1) specifically affects the periodically expressed proteins.

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CDC28 Protein Kinase, S cerevisiae Cell Cycle Cell Cycle Proteins Cyclins Cytoskeleton DNA Repair DNA Replication Gene Expression Regulation, Fungal Genes, Fungal Mitosis Oligonucleotide Array Sequence Analysis Phosphorylation Protein Interaction Mapping Protein Subunits Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription, Genetic Journal Article Research Support, Non-U.S. Gov't

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Ulrik de Lichtenberg, Lars Juhl Jensen, Søren Brunak, Peer Bork. 2005-02-04. Dynamic complex formation during the yeast cell cycle.. https://doi.org/10.1126/science.1105103

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