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A universal method for automated gene mapping.

Peder Zipperlen·Knud Nairz·Ivo Rimann·Konrad Basler·Ernst Hafen·Michael Hengartner·Alex Hajnal
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Abstract

Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual input and at comparatively low cost.

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AnimalsCaenorhabditis elegansChromosome MappingDrosophila melanogasterGenes, HelminthGenes, InsectGenomicsGenotypePolymerase Chain ReactionPolymorphism, GeneticSequence DeletionTandem Repeat SequencesJournal ArticleResearch Support, Non-U.S. Gov'tValidation Study

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Peder Zipperlen, Knud Nairz, Ivo Rimann, Konrad Basler, Ernst Hafen, Michael Hengartner, Alex Hajnal. 2005-01-17. A universal method for automated gene mapping.. https://doi.org/10.1186/gb-2005-6-2-r19

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