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PubMed · 15695805

Antibody-based proteomics for human tissue profiling.

Mathias Uhlen·Fredrik Ponten
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Abstract

Here, we describe the use of antibody-based proteomics involving the generation of protein-specific antibodies to functionally explore the human proteome. The antibodies can be used for analysis of corresponding proteins in a wide range of assay platforms, including i) immunohistochemistry for detailed tissue profiling, ii) specific affinity reagents for various functional protein assays, and iii) capture ("pull-down") reagents for purification of specific proteins and their associated complexes for structural and biochemical analyses. In this review, the use of antibodies for such analysis will be discussed with focus on the possibility to create a descriptive and comprehensive protein atlas for tissue distribution and subcellular localization of human proteins in both normal and disease tissues.

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AntibodiesHumansProtein Array AnalysisProtein Processing, Post-TranslationalProteinsProteomeProteomicsTissue DistributionJournal ArticleReview

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Mathias Uhlen, Fredrik Ponten. 2005-02-05. Antibody-based proteomics for human tissue profiling.. https://doi.org/10.1074/mcp.r500009-mcp200

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