Imaging single-channel calcium microdomains by total internal reflection microscopy.

Angelo Demuro; Ian Parker

doi:10.4067/s0716-97602004000400025

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PubMed · 15709697

Imaging single-channel calcium microdomains by total internal reflection microscopy.

Angelo Demuro·Ian Parker

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Abstract

The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic 'building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocytes.

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Animals Calcium Channels Electric Conductivity Electrophysiology Ion Channel Gating Kinetics Membrane Microdomains Membrane Potentials Microscopy, Fluorescence Oocytes Patch-Clamp Techniques Xenopus laevis Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

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Angelo Demuro, Ian Parker. 2004. Imaging single-channel calcium microdomains by total internal reflection microscopy.. https://doi.org/10.4067/s0716-97602004000400025

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