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Protein microarray using alpha-amino acids as metal tags on chips.

Supachok Sinchaikul·Fu-Ming Pan·Ching-Wen Cheng·Chi-Huey Wong·Jim-Min Fang·Min-Jen Tseng·Shui-Tein Chen
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Abstract

Procedures for synthesizing alpha-amino acids on a chip for coordination with transitional metal ions and a His-Tagged protein have been successfully developed as a stable protein microarray. Using the recombinant His-Tagged 3CL-protease (3CLpro) as a model for attachment to chips containing D-/L-Glu, Asp, Orn, Ser via different transitional metal ions, it was found that the Orn chip was the best of affinity binding and stability by which Zn2+ was the best metal ion for affinity while Co2+ was the best metal ion for stability. Thus, this protein microarray can be alternatively used as a high throughput screening method for rapid detection against SARS CoV 3CLpro and/or efficient purification of other Tagged proteins.

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Amino AcidsMetals, HeavyMolecular ConformationProtein Array AnalysisRecombinant ProteinsStructure-Activity RelationshipTime FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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Supachok Sinchaikul, Fu-Ming Pan, Ching-Wen Cheng, Chi-Huey Wong, Jim-Min Fang, Min-Jen Tseng, Shui-Tein Chen. 2005-03-01. Protein microarray using alpha-amino acids as metal tags on chips.. https://doi.org/10.1016/j.bmcl.2005.01.001

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