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Human papillomavirus type 16 E2 and E6/E7 variants.

David C Swan·Mangalathu Rajeevan·Guillermo Tortolero-Luna·Michele Follen·Ruth Ann Tucker·Elizabeth R Unger
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Abstract

OBJECTIVES: Polymorphisms in human papillomavirus (HPV) type 16 have been shown to be related to geographic areas and are broadly classified as European (E), African (Af), Asian (As), or Asian-American (AA). Certain variants have been reported as being more likely to cause cervical disease; our objectives were to identify new HPV16 polymorphisms, to determine the linkage of the E2 and E6/E7 regions and to determine the minimum sequence necessary to classify variants. METHODS: We sequenced the complete E2, E6, and E7 regions in all HPV16-positive cervical samples identified in a case-control study of pre-invasive cervical disease. RESULTS: In the 100 samples analyzed, only one new polymorphism was identified, a synonymous change, T3205A, in region E2. The frequency distribution of variants in the sample set was 37 European prototypes and 27 E-G350, 16 AA, 5 Af1, 2 Af2, 8 E-C109G, 3 E-G131G, and 2 As. As shown by others, region E7 varied much less than E6 and E2. CONCLUSIONS: In each case, E2 changes were linked to the expected E6/E7 changes, and there was no evidence for recombination. The linkage between E2 and E6/E7 allows variant classification to be based on a short E6 sequence (nt 109-350).

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Base SequenceCase-Control StudiesDNA, ViralDNA-Binding ProteinsFemaleHumansOncogene Proteins, ViralPapillomaviridaePapillomavirus E7 ProteinsPapillomavirus InfectionsPolymorphism, GeneticRepressor ProteinsTumor Virus InfectionsUterine Cervical NeoplasmsUterine Cervical DysplasiaJournal Article

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David C Swan, Mangalathu Rajeevan, Guillermo Tortolero-Luna, Michele Follen, Ruth Ann Tucker, Elizabeth R Unger. 2005. Human papillomavirus type 16 E2 and E6/E7 variants.. https://doi.org/10.1016/j.ygyno.2004.11.045

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