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Rapid microwave-assisted solid-phase glycopeptide synthesis.

Takahiko Matsushita·Hiroshi Hinou·Masaki Kurogochi·Hiroki Shimizu·Shin-Ichiro Nishimura
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Abstract

Coupling of glycosylated Fmoc-Thr or Fmoc-Ser with N-terminal amino acids on a resin proceeded smoothly under microwave irradiation for 20 min with much higher efficiency (98% yield per coupling) than found in more general conditions. Compared with a conventional protocol, the present method greatly reduces the time required for solid-phase glycopeptide synthesis from 4 days to 7 h, as is the case with the synthesis of Muc-1-related 20-residue glycopeptide carrying five core-2 trisaccharide chains. [structure: see text]

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Amino Acid SequenceAmino AcidsGlycopeptidesMicrowavesMolecular Sequence DataSerineThreonineComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Takahiko Matsushita, Hiroshi Hinou, Masaki Kurogochi, Hiroki Shimizu, Shin-Ichiro Nishimura. 2005-03-03. Rapid microwave-assisted solid-phase glycopeptide synthesis.. https://doi.org/10.1021/ol0474352

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