Streptococcus suis serotype 2 binding to extracellular matrix proteins.

Miriam Esgleas; Sonia Lacouture; Marcelo Gottschalk

doi:10.1016/j.femsle.2005.01.017

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Streptococcus suis serotype 2 binding to extracellular matrix proteins.

Miriam Esgleas·Sonia Lacouture·Marcelo Gottschalk

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Abstract

Streptococcus suis serotype 2 is a major swine and human pathogen that causes septicemia and meningitis. The ability of S. suis serotype 2 to bind to different extracellular matrix (ECM) proteins was evaluated by ELISA. All 23 strains tested bound to plasma and cellular fibronectin and collagen types I, III, and V, some to fibrin, vitronectin, and laminin, and none to the other ECM proteins tested. An unencapsulated isogenic mutant bound to ECM proteins better than its parental encapsulated strain, suggesting that the polysaccharide capsule interfered with binding. Cross-inhibition was observed between soluble plasma fibronectin and collagens in the ECM adherence assay, indicating that binding domains for both proteins exist on the same or nearby bacterial surface molecules. On the other hand, pre-incubation with plasma fibronectin increased binding to collagen IV, suggesting that S. suis might use fibronectin as a bridging molecule. The results of heat treatment and proteolytic digestion suggest that adhesins for these ECM proteins are proteinaceous in nature.

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Animals Bacterial Adhesion Collagen Extracellular Matrix Proteins Fibronectins Humans In Vitro Techniques Protein Binding Serotyping Streptococcus suis Swine Journal Article Research Support, Non-U.S. Gov't

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Miriam Esgleas, Sonia Lacouture, Marcelo Gottschalk. 2005-03-01. Streptococcus suis serotype 2 binding to extracellular matrix proteins.. https://doi.org/10.1016/j.femsle.2005.01.017

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