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Endothelialized microvasculature based on a biodegradable elastomer.

Christina Fidkowski·Mohammad R Kaazempur-Mofrad·Jeffrey Borenstein·Joseph P Vacanti·Robert Langer·Yadong Wang
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Abstract

Vital organs maintain dense microvasculature to sustain the proper function of their cells. For tissue- engineered organs to function properly, artificial capillary networks must be developed. We have microfabricated capillary networks with a biodegradable and biocompatible elastomer, poly(glycerol sebacate) (PGS). We etched capillary patterns onto silicon wafers by standard micro-electromechanical systems (MEMS) techniques. The resultant silicon wafers served as micromolds for the devices. We bond the patterned PGS film with a flat film to create capillary networks that were perfused with a syringe pump at a physiological flow rate. The devices were endothelialized under flow conditions, and part of the lumens reached confluence within 14 days of culture. This approach may lead to tissue-engineered microvasculature that is critical in vital organs engineering.

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Biocompatible MaterialsBiodegradation, EnvironmentalCell AdhesionCell Culture TechniquesCell LineCell ProliferationElastomersEndothelium, VascularHumansImplants, ExperimentalMicrocirculationPerfusionSiliconSurface PropertiesTissue EngineeringUmbilical VeinsJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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BibTeXRIS

Christina Fidkowski, Mohammad R Kaazempur-Mofrad, Jeffrey Borenstein, Joseph P Vacanti, Robert Langer, Yadong Wang. Endothelialized microvasculature based on a biodegradable elastomer.. https://doi.org/10.1089/ten.2005.11.302

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