PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 15742059

Microflow electroorganic synthesis without supporting electrolyte.

Roberto Horcajada·Masayuki Okajima·Seiji Suga·Jun-ichi Yoshida
View original source ↗Export metadata ↓

Abstract

Anodic methoxylation of several organic compounds has been successfully achieved in the absence of intentionally added supporting electrolyte using an electrochemical microflow system.

Explore related subjects

ElectrochemistryMicrofluidic Analytical TechniquesMicrofluidicsMolecular StructureOrganic ChemicalsPorositySensitivity and SpecificitySurface PropertiesTime FactorsJournal ArticleResearch Support, Non-U.S. Gov't

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Roberto Horcajada, Masayuki Okajima, Seiji Suga, Jun-ichi Yoshida. 2005-01-20. Microflow electroorganic synthesis without supporting electrolyte.. https://doi.org/10.1039/b417388k

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2005

Enzyme-like kinetics of ferryloxy myoglobin formation in films on electrodes in microemulsions.

Covalently linked films of the ferric heme protein myoglobin and poly-L-lysine on pyrolytic graphite electrodes reacted with tert-butylhydroperoxide (tBuOOH) to form ferryloxy protein species according to Michaelis-Menten enzyme kinetics. Rotating disk voltammetry data obtained in microemulsions, micellar solution, and buffers revealed a strong influence of water phase acidity on kinetic parameters. Microemulsion and surfactant type had a much smaller influence on reaction kinetics, possibly because the reaction takes place entirely in a water environment surrounding Mb in the films in all fluids. A large apparent Michaelis kcat in microemulsions with neutral water phases was offset by much weaker binding as shown by larger protein-substrate dissociation constants (Km). Acidic SDS microemulsions and pH 2 buffer provided the most efficient reaction conditions as judged by the ratio kcat/Km. Apparent kinetic constants are most likely governed by acidity-controlled protein conformations and their binding with tBuOOH in the intermediate protein-substrate complex.

Electrochemistry↗
PubMed2005

Determination of human serum albumin using aurothiomalate as electroactive label.

A new electroactive label has been used to monitor immunoassays in the determination of human serum albumin (HSA) using glassy-carbon electrodes as supports for the immunological reactions. The label was a gold(I) complex, sodium aurothiomalate, which was bound to rabbit IgG anti-human serum albumin (anti-HSA-Au). The HSA was adsorbed on the electrode surface and the immunological reaction with gold-labelled anti-HSA was then performed for one hour by non-competitive or competitive procedures. The gold(I) bound to the anti-HSA was electrodeposited in 0.1 mol L-1 HCl at -1.00 V for 5 min then oxidised in 0.1 mol L-1 H2SO4 solution at +1.40 V for 1 min. Silver electrodeposition at -0.14 V for 1 min followed by anodic stripping voltammetry were then performed in aqueous 1.0 mol L-1 NH(3)-2.0x10(-4) mol L-1 AgNO3. For both non-competitive and competitive formats, calibration plots in the ranges 5.0x10(-10) to 1.0x10(-8) mol L-1 and 1.0x10(-10) to 1.0x10(-9) mol L-1 HSA, respectively, with estimated detection limits of 1.5x10(-10) mol L-1 (10 ng mL-1) and 1.0x10(-10) mol L-1 (7 ng mL-1), respectively, were obtained. Levels of HSA in two healthy volunteer urine samples were also evaluated, using both immunoassay formats.

Electrochemistry↗