Changing cell fate by nuclear reprogramming.

Stina Simonsson; J B Gurdon

doi:10.4161/cc.4.4.1581

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PubMed · 15753660

Changing cell fate by nuclear reprogramming.

Stina Simonsson·J B Gurdon

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Abstract

The differentiated state of somatic cells is remarkably stable, but nuclear transfer can efficiently override the stability of cell differentiation by nuclear reprogramming. Genes that were silent in the differentiated state are activated, whereas genes specific for the differentiated state are switched off during the reprogramming process. The epigenetic changes that occur after nuclear transfer to Xenopus oocytes involve chromatin remodelling and DNA demethylation. In particular, we have reported that reactivation of the mouse stem cell specific gene oct-4 depends on demethylation of CpG:s in the proximal oct-4 promoter. Here we discuss molecular mechanisms of nuclear reprogramming, with special emphasis on DNA demethylation.

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Animals Cell Cycle Cell Differentiation Chromatin Cloning, Organism DNA Methylation Epigenesis, Genetic Gene Silencing Humans Mice Nuclear Matrix Octamer Transcription Factor-3 Oocytes Promoter Regions, Genetic Stem Cells Transcription, Genetic Xenopus Journal Article Review

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Stina Simonsson, J B Gurdon. 2005-05-01. Changing cell fate by nuclear reprogramming.. https://doi.org/10.4161/cc.4.4.1581

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