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Model system using coliphage phi X174 for testing virus removal by air filters.

M L Rapp·T Thiel·R J Arrowsmith
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Abstract

Short-term (15-min-duration) and long-term (5- to 6-day-duration) test procedures have been developed for determining the efficiency of the removal of bacteriophage phi X174 by air-sterilizing filters. These procedures were sensitive enough to measure a 10(8)-fold reduction in the number of bacteriophage. A filter commonly used in industrial air sterilizations (Domnick-Hunter Bio-X borosilicate glass) effected a 10(8)-fold removal of viable phage in both short-term and long-term tests. A prototype low-flux, hollow-fiber membrane gave similar results; however, a prototype high-flux, hollow-fiber membrane removed only about 99.999% of the bacteriophage in short-term tests.

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ColiphagesEvaluation Studies as TopicFiltrationJournal ArticleResearch Support, Non-U.S. Gov't

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M L Rapp, T Thiel, R J Arrowsmith. 1992. Model system using coliphage phi X174 for testing virus removal by air filters.. https://doi.org/10.1128/aem.58.3.900-904.1992

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