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PubMed · 15776154

Sequential affinity chromatography miniaturized within a "lab-on-valve" system.

Holger Erxleben·Jaromir Ruzicka
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Abstract

An automatically renewable microcolumn, subjected to operation by programmable flow, is presented and for the first time used for separation and quantification of biomolecules on Sepharose Protein A beads, with absorbance measurement at 280 nm and a detection limit of 6 ng mouse IgG microL(-1).

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AnimalsCattleChromatography, AffinityFlow Injection AnalysisImmunoglobulin GMiceMicrochemistrySerum Albumin, BovineJournal Article

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Holger Erxleben, Jaromir Ruzicka. 2005-03-03. Sequential affinity chromatography miniaturized within a "lab-on-valve" system.. https://doi.org/10.1039/b501039j

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