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Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.

Michelle A Digman·Parijat Sengupta·Paul W Wiseman·Claire M Brown·Alan R Horwitz·Enrico Gratton
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Abstract

Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.

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AlgorithmsAnimalsBiophysicsCell LineCricetinaeDiffusionGreen Fluorescent ProteinsImage Interpretation, Computer-AssistedImage Processing, Computer-AssistedLasersMicroscopy, ConfocalMicroscopy, FluorescenceModels, StatisticalNormal DistributionPhotonsTime FactorsJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Michelle A Digman, Parijat Sengupta, Paul W Wiseman, Claire M Brown, Alan R Horwitz, Enrico Gratton. 2005-03-25. Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.. https://doi.org/10.1529/biophysj.105.061788

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