High-resolution functional proteomics by active-site peptide profiling.

Eric S Okerberg; Jiangyue Wu; Baohong Zhang; Babak Samii; Kelly Blackford; David T Winn; Kevin R Shreder; Jonathan J Burbaum; Matthew P Patricelli

doi:10.1073/pnas.0501205102

PubMed · 15795380

High-resolution functional proteomics by active-site peptide profiling.

Eric S Okerberg·Jiangyue Wu·Baohong Zhang·Babak Samii·Kelly Blackford·David T Winn·Kevin R Shreder·Jonathan J Burbaum·Matthew P Patricelli

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Abstract

Characterization and functional annotation of the large number of proteins predicted from genome sequencing projects poses a major scientific challenge. Whereas several proteomics techniques have been developed to quantify the abundance of proteins, these methods provide little information regarding protein function. Here, we present a gel-free platform that permits ultrasensitive, quantitative, and high-resolution analyses of protein activities in proteomes, including highly problematic samples such as undiluted plasma. We demonstrate the value of this platform for the discovery of both disease-related enzyme activities and specific inhibitors that target these proteins.

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Animals Binding Sites Electrophoresis, Capillary Mice Peptide Mapping Peptides Proteomics Serine Endopeptidases Journal Article

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Eric S Okerberg, Jiangyue Wu, Baohong Zhang, Babak Samii, Kelly Blackford, David T Winn, Kevin R Shreder, Jonathan J Burbaum, Matthew P Patricelli. 2005-03-28. High-resolution functional proteomics by active-site peptide profiling.. https://doi.org/10.1073/pnas.0501205102

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