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PubMed · 15797876

Specific IgE to colophony?

Joanne Elms·David Fishwick·Edward Robinson·Sherwood Burge·Vicky Huggins·Chris Barber·Nerys Williams·Andrew Curran
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Abstract

BACKGROUND: Colophony (rosin) is a natural product obtained from coniferous trees. It is used in a diverse range of products such as adhesives, ink, paints and soldering fluxes. Some workers exposed to colophony during soldering can develop occupational asthma; at present, no specific IgE test is available to assess sensitization to colophony. METHODS: Serum samples were obtained from exposed symptomatic individuals (n = 7), some with a likely diagnosis of occupational asthma, exposed asymptomatic individuals (n = 10) and unexposed individuals (n = 11). Serum was tested for specific IgE antibodies against a protein extract produced following in vitro challenge of mono-mac-6 cells with colophony extract. RESULTS: Serum from exposed symptomatic individuals showed increased binding of specific IgE antibodies to a range of colophony-cell protein conjugates [29% (2/7) of samples tested when cut-off > 0.1 or 86% (6/7) of samples tested when cut-off > 0%] compared with both the exposed asymptomatic [0% when cut-off > 0.1, or 20% when cut-off > 0% (2/10)] and the non-exposed control populations [0% when cut-off > 0.1, or 27% when cut-off > 0% (3/11)]. CONCLUSIONS: This novel approach for the production of conjugates to assess sensitization to colophony was able to detect specific IgE in colophony-exposed workers with a likely diagnosis of occupational asthma.

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AdultAsthmaHumansImmunoglobulin EMiddle AgedOccupational DiseasesRadioallergosorbent TestResins, PlantJournal Article

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Joanne Elms, David Fishwick, Edward Robinson, Sherwood Burge, Vicky Huggins, Chris Barber, Nerys Williams, Andrew Curran. 2005-03-29. Specific IgE to colophony?. https://doi.org/10.1093/occmed%2Fkqi047

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