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Complement receptors regulate lipopolysaccharide-induced T-cell stimulation.

Ziya Kaya·Theresa Tretter·Jens Schlichting·Florian Leuschner·Marina Afanasyeva·Hugo A Katus·Noel R Rose
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Abstract

Complement receptors type 1 and 2 (CR1 (CD35)/CR2 (CD21)) are known to enhance the adaptive immune response. In mice, CR1/CR2 are expressed on B cells, follicular dendritic cells, and activated granulocytes. Recently, we showed that a subset of CD44high and CD62Llow T cells also expresses CR1 and CR2. We now report that CR1/CR2 are detectable on both CD4+ and CD8+ subsets of T cells. Lipopolysaccharide (LPS) from Gram-negative bacteria causes polyclonal activation of B cells and stimulation of macrophages and other antigen-presenting cells. We further demonstrate that LPS induced marked up-regulation of CD25 and CD69 on T cells from CR1/CR2 sufficient (Cr+/+), but significantly lower up-regulation on T cells from CR1/CR2 deficient (Cr-/-) mice. These findings point to a novel mechanism by which CR1/CR2 modulates the activation of T cells by LPS.

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AnimalsAntigens, CDAntigens, Differentiation, T-LymphocyteB-LymphocytesCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell SeparationCells, CulturedFlow CytometryLectins, C-TypeLipopolysaccharidesLymphocyte ActivationMacrophagesMaleMiceMice, Inbred C57BLReceptors, ComplementReceptors, Complement 3bReceptors, Complement 3dReceptors, Interleukin-2T-LymphocytesCD69 AntigensJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Ziya Kaya, Theresa Tretter, Jens Schlichting, Florian Leuschner, Marina Afanasyeva, Hugo A Katus, Noel R Rose. 2005. Complement receptors regulate lipopolysaccharide-induced T-cell stimulation.. https://doi.org/10.1111/j.1365-2567.2004.02113.x

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