NMR experiments on aligned samples of membrane proteins.

A A De Angelis; D H Jones; C V Grant; S H Park; M F Mesleh; S J Opella

doi:10.1016/s0076-6879(05)94014-7

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PubMed · 15808228

NMR experiments on aligned samples of membrane proteins.

A A De Angelis·D H Jones·C V Grant·S H Park·M F Mesleh·S J Opella

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Abstract

NMR methods can be used to determine the structures of membrane proteins. Lipids can be chosen so that protein-containing micelles, bicelles, or bilayers are available as samples. All three types of samples can be aligned weakly or strongly, depending on their rotational correlation time. Solution NMR methods can be used with weakly aligned micelle and small bicelle samples. Solid-state NMR methods can be used with mechanically aligned bilayer and magnetically aligned bicelle samples.

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Electrophoresis, Polyacrylamide Gel Lipid Bilayers Magnetic Resonance Spectroscopy Membrane Proteins Protein Structure, Secondary Time Factors Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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A A De Angelis, D H Jones, C V Grant, S H Park, M F Mesleh, S J Opella. 2005. NMR experiments on aligned samples of membrane proteins.. https://doi.org/10.1016/s0076-6879(05)94014-7

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