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In vitro immune response of human peripheral lymphocytes. II. Properties and functions of concanavalin A-induced suppressor T cells.

T Kuritani·T Hirano·T Kishimoto·Y Yamamura
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Abstract

Con A-stimulation of human peripheral T lymphocytes induced both suppressor and helper T cells. ConA-generated suppressor T cells inhibited PWM-induced IgG and IgM production in PBL. Lower concentrations of Con A (0.5 micrograms/ml) or shorter incubation periods (6 to 24 hr) induced mainly helper T cells, while higher concentrations of Con A (10 micrograms/ml) or longer incubation periods (at least 48 hr) induced suppressor T cells. Con A-generated suppressor T cells were sensitive to mitomycin treatment and exerted their suppressor function on the early phase of differentiation and/or proliferation of B cells but not on the final differentiation of B cells to Ig-producing cells. The identity of the MHC was not required for the expression of suppressor function. Suppressor T cells competed with helper T cells in PWM-induced Ig-production of PBL. This experimental system can be applied to estimate the regulatory function of T cells in several disease states.

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B-LymphocytesConcanavalin ADose-Response Relationship, ImmunologicHumansImmunoglobulin GImmunoglobulin MImmunoglobulinsMitomycinsT-LymphocytesT-Lymphocytes, RegulatoryJournal Article

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T Kuritani, T Hirano, T Kishimoto, Y Yamamura. 1979. In vitro immune response of human peripheral lymphocytes. II. Properties and functions of concanavalin A-induced suppressor T cells.. https://doi.org/10.1111/j.1348-0421.1979.tb00455.x

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