PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 15820959

An improved method for lupus anticoagulant detection.

Izaskun Alonso·Francine Vandermijnsbrugge·Els Vanremoortele·Cristophe Bradstreet·Andre Efira·Brigite Cantinieaux
View original source ↗Export metadata ↓

Abstract

The diagnosis of lupus anticoagulant (LA) becomes difficult when there is a weak titer of LA antibodies. This study demonstrates that 20 minutes' incubation increases the sensitivity of the mixing test to diagnose LA by 23% and to suspect the antibodies by 66%.

Explore related subjects

Blood Coagulation TestsHumansLupus Coagulation InhibitorSensitivity and SpecificityLetter

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Izaskun Alonso, Francine Vandermijnsbrugge, Els Vanremoortele, Cristophe Bradstreet, Andre Efira, Brigite Cantinieaux. 2005. An improved method for lupus anticoagulant detection.. https://pubmed.ncbi.nlm.nih.gov/15820959/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2007

In vitro kinetics of factor VIII activity in patients with mild haemophilia A and a discrepancy between one-stage and two-stage factor VIII assay results.

In some mild haemophilia A patients (discrepant haemophilia), factor VIII coagulant activity (FVIII:C) levels, by one-stage assay are more than double than those by two-stage assay. This may be due to the longer incubation times (10-12 min) in the two-stage assay. This study aimed to determine the time course of the activation phase of the two-stage assay, using both classical coagulation and chromogenic detection methods. In both systems, for equivalent patients (equivalent FVIII:C levels by one-stage and two-stage assays, n = 6, all different mutations), similar FVIII:C results were obtained with short- or long-incubation times. In contrast, plasma from discrepant patients (n = 8, five different mutations) showed higher FVIII:C at shorter incubation times than after longer incubation times. In the chromogenic assay, FVIII:C levels were higher after incubation for 2 min (23-56%, mean 41%) than after 10 min (19-41%, mean 29%). In the classical coagulation assay, FVIII:C levels were higher at shorter incubation times (21-64%, mean 37%) than with the longer incubation times usually used (13-29%, mean 23%). These time-course experiments have verified that the longer incubation time used in the two-stage assay is at least partly responsible for the lower FVIII:C measured by that assay in discrepant haemophilia.

Blood Coagulation Tests↗
PubMed2007

Von Willebrand disease within the collective of haemophilic patients as reason for unexpected bleeding episodes.

In the treatment of haemophilia A and B, recombinant coagulation factors are increasingly replacing plasma-derived factor VIII and IX concentrates. Although provided with replacement therapy, individual patients may exhibit bleeding episodes, which are difficult to control. These bleeds may be caused by von Willebrand disease (VWD) as an additional underlying coagulation disorder. We report in the present study our experience that in the collective of haemophilic patients, VWD must be anticipated at least with the same order of magnitude as it appears in a normal healthy population. Among the patients at our treatment centre, two patients (1.5%) were identified as suffering from VWD in addition to haemophilia A. In the collective of haemophilia B patients at our centre, three patients (10%) with VWD were found. Two of these patients exhibited unexpected severe bleeding episodes, which could only satisfactorily be controlled by the administration of Haemate-P or DDAVP supplementary to the recombinant coagulation factor concentrate.

Blood Coagulation Tests↗